such an action will guarantee many won't consider reading the story until after it's finished, if they remember to bookmark it.
I'm not so sure about that. There are readers who read stories as they post, and those who only read completed stories, but I doubt anyone would refuse to read a story until a glossary is posted.
In terms of a Character List, it's best not to include one unless there are a LOT of characters who can be easily confused or easily forgotten (i.e. not seen in the story for multiple chapters).
In terms of a glossary, it's generally best to explain terms in context (ex: "Joe was an MP (Military Police)" or "Joe was an MP, tasked with keeping order on the base"), rather than requiring readers to keep jumping in and out of the story.
Yes, Tom Clancy did include a Glossary, but that was in a printed book, where he could include the full glossary in the full book, which is different than posting a story a chapter at a time.
In general, unexpected files get read less. Prologues typically get about 40 to 60% reads (depending on genres and whether readers are used to encountering them). Prefaces, which explain aspects of the story, get even fewer reads (maybe 20%). Character Lists get more, but those are typically overcounted due to read-aheads and readers mistakenly reading them as part of the story (something you don't want).
You can train readers what to expect by consistently offering similar content in each of your stories, but it's a slow process, and you can't expect new readers (always a valued target group) to know what to read and what not to. Mostly what gets read is related to the established genres (ex. Prologues are typical for sci-fi and historical dramas, but not for romances or mysteries).
If you ever want to discuss when it's helpful to include one of these chapters, feel free to ask us, as many of us have experience with a variety of alternate chapters.